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Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli

✍ Scribed by David E Drew; Gunnar von Heijne; Pär Nordlund; Jan-Willem L de Gier


Book ID
117103503
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
213 KB
Volume
507
Category
Article
ISSN
0014-5793

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We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,