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Green fluorescent protein as a second selectable marker for selection of high producing clones from transfected CHO cells

✍ Scribed by Y.Gloria Meng; Jie Liang; Wai Lee Wong; Vanessa Chisholm

Book ID
117523967
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
229 KB
Volume
242
Category
Article
ISSN
0378-1119

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## Abstract In order to improve the current techniques of cell cultivation in the absence of serum, we have developed a protein‐free transfection protocol for CHO cells, based on the Nucleofector™ technology. After starting with a heterogeneous pool of primary transfectants which express the fusion