Grapefruit Juice Selectively Modifies Cyclosporin a Metabolite Patterns in Renal Transplant Patients

Liquid chromatography–tandem mass spectr

Liquid chromatography–tandem mass spectrometry method for simultaneous determination of cyclosporine A and its three metabolites AM1, AM9 and AM4N in whole blood and isolated lymphocytes in renal transplant patients

✍ Hana Brozmanová; Ilona Peřinová; Petra Halvová; Milan Grundmann 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB

## Abstract A LC‐MS/MS method was developed and validated for the determination of cyclosporine A (CsA) and its three phase 1 metabolites AM1, AM9, and AM4N in whole blood and lymphocytes isolated on the Histopaque gradient. 200 μL of whole blood was precipitated with 10 mol/L zinc sulfate in aceto