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Granulopoietic differentiation in long-term bone marrow cultures from children with congenital neutropenia

✍ Scribed by Dr. Laure Coulombel; Nada Morardet; Florence Veber; Christine Leroy; Francoise Mielot; Alain Fisher; Françoise Teillet-Thiebaud; Gil Tchernia; Claude Griscelli; Claude Parmentier


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
504 KB
Volume
27
Category
Article
ISSN
0361-8609

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Murine long-term bone marrow cultures (LTBMCs) were used to generate hematopoietic cells free from marrow stromal cells. These progenitor cells were treated with GM-CSF (5 U/ml) with or without rat bone osteocalcin or rat serum albumin in either a-MEM with 2% heat-inactivated horse serum alone (a) o