Granulocyte-macrophage Colony Stimulating Factor (GM-CSF) and Interleukin 8 (IL-8) Production by Human Bronchial Epithelial Cells (HBEC) in Asthmatics and Controls. Lack of In Vitro Effect of Salbutamol Compared to Sodium Nedocromil
✍ Scribed by F. Gormand; S. Cheria-Sammari; R. Aloui; B. Guibert; D. Malicier; M. Perrin-Fayolle; M. Lagarde; Y. Pacheco
- Publisher
- Elsevier
- Year
- 1995
- Tongue
- English
- Weight
- 354 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0952-0600
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✦ Synopsis
SUMMARY: The bronchial epithelium produces cytokines that could contribute to inflammatory events in airways. In this study we determined the basal and TNF (\alpha) stimulated productions of GM-CSF and IL-8 by human bronchial epithelial cells (HBEC) collected from 12 control and six asthmatic patients. Spontaneous and TNF (\alpha) induced GM-CSF or IL-8 released levels increased significantly with time. Epithelial cells from asthmatic patients spontaneously released high levels of GM-CSF ((24 \mathrm{~h})). TNF (\alpha) potentiated GM-CSF and IL-8 release in control subjects and only the IL-8 production in asthmatics. Nedocromil sodium, an antiinflammatory drug, and salbutamol, a beta2-agonist, are commonly used in asthma. They were evaluated on the spontaneous and TNF-induced expression of GM-CSF and IL-8 in cultured bronchial epithelial cells. Nedocromil sodium, at the concentration of (10^{-6} \mathrm{M}), reduced the TNF-induced increase in GM-CSF but not the IL-8 release. Salbutamol, at the concentration of (10^{-6}) or (10^{-5} \mathrm{M}), did not affect the constitutive or stimulated production of both cytokines.