Grafting of silica with a hydrophilic triol acrylamide polymer via surface-initiated “grafting from” method for hydrophilic-interaction chromatography

✍ Scribed by Xi-Tian Peng; Bi-Feng Yuan; Yu-Qi Feng

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 232 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201100570

✦ Synopsis

Abstract

A novel hydrophilic polymer‐coated silica sorbent has been prepared using the radical “grafting from” polymerization method through surface‐bound azo initiators for hydrophilic‐interaction chromatography (HILIC). The azo groups were introduced to the surface of silica gel through the reaction with amino groups on the surface of silica gel with 4,4′‐azobis(4‐cyanopentanoic acid chloride) (ACVC). The resultant azo‐immobilized silica gel served as surface initiator to polymerize hydrophilic triol acrylamide monomer N‐acryloyltris(hydroxymethyl) aminomethane (NA) in methanol to get hydrophilic polymer‐coated silica sorbent. The obtained poly(NA)‐coated silica (pNA‐sil) was characterized by Fourier transform infrared spectroscopy (FT‐IR), elemental analysis (EA), and nitrogen sorption porosimetry (NSP). Then the pNA‐sil was packed into the stainless‐steel column and evaluated in high‐performance liquid chromatography (HPLC). Good chromatographic performance for the separation of peptides and nucleosides was obtained under HILIC mode. The results indicated that the pNA‐sil stationary phase behaved as mixed‐mode retention mechanisms of hydrophilic and ionic interactions. Furthermore, the pNA‐sil phase was used to separate tryptic digest of β‐casein and our results showed that more than 12 peptides peaks were resolved and well distributed within the elution window. Finally, the pNA‐sil stationary phase was demonstrated to possess remarkable reproducibility and stability. Taken together, the pNA‐sil stationary phase prepared in the current study offers a potential application in proteomics study.