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Gradient-enhanced proton–fluorine NOE experiments

✍ Scribed by J. T. Gerig

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
112 KB
Volume
37
Category
Article
ISSN
0749-1581

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✦ Synopsis

Uses of pulsed field gradients in the detection of 1 Hf 19 Fg nuclear Overhauser effects in biological systems were examined in experiments wherein gradients were used for coherence selection and recall, as well as simply for the removal of unwanted magnetization components. Strategies for selective detection of these NOEs when the fluorine resonance is broad are proposed. Detection of 1 Hf 19 Fg nuclear Overhauser effects in a carbonic anhydrasefluorinated inhibitor complex demonstrates the methods considered and shows the superiority of the "inverse"approach to detection of fluorine-proton 1 Hf 19 Fg NOEs in macro-molecular systems.

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