✦ LIBER ✦

Gonadotropin stimulation of cyclic AMP levels in Chinese Hamster Ovary cells in culture

✍ Scribed by Daniele Evain; Wayne B. Anderson

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 409 KB
Volume: 99
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040990116

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

When Chinese Hamster Ovary (CHO) cells, incubated in serum‐free medium, are exposed to gonadotropins a transient increase in the intracellular concentration of cyclic AMP is observed. Maximum accumulation of cyclic AMP is noted 30 minutes after addition of either human chorionic gonadotropin (hCG) or follicle stimulating hormone (FSH). Within one to two hours after hormone addition, the intracellular concentrations of cyclic AMP have returned to basal levels. The enhancement of intracellular cyclic AMP levels by hCG is hormone concentration dependent, with maximal stimulation observed at 10 μg/ml hCG.

The exogenous addition of gonadotropins also slows the growth rate of CHO cells. This effect on growth seems to be mediated through cyclic AMP since the growth rate of a mutant of CHO cells defective in the catalytic subunit of cyclic AMP dependent protein kinase is only slightly decreased.

📜 SIMILAR VOLUMES

Gonadotropin stimulation of pregnenolone

Gonadotropin stimulation of pregnenolone metabolism in Chinese Hamster Ovary cells in culture

✍ Daniele Evain; Wayne B. Anderson; Jose M. Saez 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 457 KB

## Abstract To determine if Chinese Hamster Ovary (CHO) cells in culture are able to metabolize steroids, CHO cells were incubated in defined medium with [^14^C]pregnenolone. As shown, [^14^C]pregnenolone is metabolized to progesterone and other δ^5^3β steroids; this steroidogenic response is appre

Apoptosis in batch cultures of Chinese H

Apoptosis in batch cultures of Chinese Hamster Ovary cells

✍ J. Goswami; A. J. Sinskey; H. Steller; G. N. Stephanopoulos; D. I. C. Wang 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 226 KB 👁 2 views

One of the main problems in the culture of Chinese Hamster Ovary (CHO) cells continues to be the inability to maintain the viability of the cultures over an extended period of time. The rapid decline in viability at the end of the culture is exacerbated by the absence of serum. In trying to reduce t

Control of starvation-induced apoptosis

Control of starvation-induced apoptosis in Chinese hamster ovary cell cultures

✍ Laurent Simon; M. Nazmul Karim 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 214 KB 👁 2 views

A comparison of induced sister chromatid

A comparison of induced sister chromatid exchange levels in chinese hamster ovary cells and cultured human lymphocytes

✍ Morgan, William F. ;Crossen, Peter E. 📂 Article 📅 1982 🏛 Wiley (John Wiley & Sons) ⚖ 389 KB 👁 1 views

Tetracycline-Regulated overexpression of

Tetracycline-Regulated overexpression of glycosyltransferases in Chinese hamster ovary cells

✍ Pablo Umaña; Joël Jean-Mairet; James E. Bailey 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 379 KB 👁 2 views

The glycosylation patterns of recombinant therapeutic glycoproteins can be engineered by overexpression of glycosyltransferases in the host cells used for glycoprotein production. Most prior glycosylation engineering experiments have involved constitutive expression of cloned glycosyltransferases. H

Metabolic effects on recombinant interfe

Metabolic effects on recombinant interferon-γ glycosylation in continuous culture of Chinese hamster ovary cells

✍ Gregg B. Nyberg; R. Robert Balcarcel; Brian D. Follstad; Gregory Stephanopoulos; 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 199 KB 👁 1 views

Asparagine linked (N-linked) glycosylation is an important modification of recombinant proteins, because the attached oligosaccharide chains can significantly alter protein properties. Potential glycosylation sites are not always occupied with oligosaccharide, and site occupancy can change with the