Gonadotropin action on androgen synthesis in short-term incubation of explants and dispersed cells of the testis ofXenopus laevis

✍ Scribed by Lecouteux, Alain

• Book ID: 102890570
• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 642 KB
• Volume: 245
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402450207

✦ Synopsis

Testis cells of the toad Xenopus laeuis were dissociated with collagenase and the cell suspension was enriched for steroidogenic cells by Percoll gradients. Results suggested that cells should be preincubated during a 6-h period before stimulation with gonadotropin. Our results indicate that a 2-h incubation period with gonadotropin was necessary to obtain a significant response. Furthermore, the cells can be maintained in a functional state longer than mammalian testis cells. Different gonadotropins were used to stimulate androgen production, and their effects were compared in both dissociated cells and testicular explants. Cells were more sensitive to luteinizing hormone (LH) and follicle stimulating hormone (FSH) than the explants (ED50 LH = 0.041 i 0.003 p g for cells and 0.097 & 0.002 p g for explants: ED5* FSH = 0.41 0.03 pg for cells and 0.63 f 0.03 p g for