Gonadal differentiation and secretions of estradiol and testosterone of the ovaries ofRana catesbeiana tadpoles treated with 4-hydroxyandrostenedione
✍ Scribed by Yu, Nai-Wen ;Hsu, Chih-Yun ;Ku, Hung-Hai ;Chang, Li-Tze ;Liu, Hwan-Wun
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 763 KB
- Volume
- 265
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Laparotomized female tadpoles of Rana catesbeiana at TK stages X–XII, about 9 months old, were implanted intraperitoneally with empty capsules or capsules containing 4‐hydroxyandrostenedione (4‐OHA), known as an aromatase inhibitor in vertebrates. Histology, gonosomatic index, and secretions of estradiol (E~2~) and testosterone (T) of the ovaries were investigated. Three months after the treatment, histological examination revealed various degrees of sex reversal in the ovaries treated with 4‐OHA and 79% (57 in 72) were transformed into testes. The ovaries of control tadpoles, however, displayed normal histological appearance. Radioimmunoassay showed that secretion of E~2~ was decreased while that of T was increased in 4‐OHA treated ovaries. The gonosomatic index displayed a decline tendency from control females through experimental animals to untreated control males. These results indicated that activity of aromatase in the ovaries was inhibited by 4‐OHA, resulting in accumulation of T which induced transformation of the ovaries into testes. © 1993 Wiley‐Liss, Inc.