A Continuous Spectrophotometric Assay fo
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M. Pineda; P. Piedras; J. Cardenas
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Article
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1994
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Elsevier Science
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English
โ 475 KB
A spectrophotometric assay for ureidoglycolase activity (both ureidoglycolate lyase and hydrolase), based on the reduction of glyoxylate to glycolate catalyzed by glyoxylate reductase or lactate dehydrogenase with the stoichiometric and continuous NADH oxidation, is described. The assay has been opt