Chymotrypsin modified with polyethylene glycol was successfully used for peptide synthesis in organic solvents. The benzene-soluble modified enzyme readily catalyzed both aminolysis of N-benzoyl-L-tyrosine p-nitroanilide and synthesis of N-benzoyl-L-tyrosine butylamide in the presence of trace amoun
✦ LIBER ✦
Glycosidases in organic solvents: II. Transgalactosylation catalysed by polyethylene glycol-modified β-galactosidase
✍ Scribed by Jean E. Beecher; Anthony T. Andrews; Evgeni N. Vulfson
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 496 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0141-0229
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Enzyme-Lipid Complex. Part 11. Effective Transgalactosylation Catalyzed by a Lipid-Coated β-D-Galactosidase in Organic Solvents. -A lipid-coated β-D-galactosidase is prepared, which is soluble in most organic solvents, but insoluble in aqueous solution. This enzyme is an efficient transgalactosylat