✦ LIBER ✦

Glycohydrolase contamination of commercial enzymes frequently used in the preparation of fungal cell walls

✍ Scribed by Thomas E. Davis Jr.; Judith E. Domer

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 584 KB
Volume: 80
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90683-2

No coin nor oath required. For personal study only.

✦ Synopsis

Commercial enzyme preparations frequently used in the preparation of fungal cell walls, viz., proteases, a lipase, and a phosphatase, were examined for the presence of contaminating glycohydrolase activity, since such activity could result not only in the removal of cytoplasmic constituents but also in the removal of portions of the wall itself. Glucosidase activities were detected in a protease of fungal origin, in a lipase from wheat germ, and in a phosphatase from potatoes. Additionally.

two commercial protease preparations from Streptomyces griseus contained p-1,3-glucanase activity in significant amounts, a third contained trace amounts of the glucanase. but a fourth was totally free of glycohydrolase activity. The protease preparations from S. griseus released laminaribiose from yeast-phase cell walls of Histoplasma capsalatum 593 CopyrIght im 1977 by Academic Press. Inc. All rIghI% ot rrproduct~~n I" any form resened.

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