Glycogen synthesis by cell-free extracts from the dimorphic yeastCandida albicans

✍ Scribed by Peter Orlean

• Book ID: 104754446
• Publisher: Springer Netherlands
• Year: 1984
• Tongue: English
• Weight: 370 KB
• Volume: 50
• Category: Article
• ISSN: 0003-6072
• DOI: 10.1007/bf00394647

✦ Synopsis

A particulate glucosyltransferase prepared from budding and filamentous cultures of Candida albicans used uridine diphosphate glucose as sole glucosyl donor in a reaction (measured by following the incorporation of[ 14C]-glucose from UDP [14C]-glucose into polymer) stimulated by glucose-6-phosphate and inhibited by adenosine triphosphate and guanosine triphosphate. The radiolabelled reaction product was solubilized by s-amylase, and, on oxidation with periodate followed by reduction with borohydride and acid hydrolysis, yielded erythritol and glycerol in the ratio of 4 to 1. The radiolabelled glucosyl residues were attached to an endogenous acceptor of high molecular weight.