Glycogen metabolizing enzyme activities in the developing rat liver
β Scribed by Ramji L. Khandelwal
- Book ID
- 107770342
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 756 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0020-711X
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Alloxan diabetes induced in white rats by intraperitoneal injection of alloxan-monohydrate (15 mg/100 g body weight) was used to study changes in the glycogen phosphorylase a and b, phosphoprotein phosphatases and hexokinase activities under insulin deficiency conditions. Among the enzymes studied,
## Abstract Parenchymal cells were isolated from adult rat liver with an enzyme perfusion technique. The singleβcell suspension, representing 40β50% of the liver's hepatocytes was suspended in medium and maintained in primary culture for up to four days. The cells were found to carry out glycogen s