Glycerophosphorylcholine phosphocholine phosphodiesterase activity during the differentiation of glial progenitor cells

✍ Scribed by M. Monge; J. Yuan; F. Cabon; B. Zalc; J. N. Kanfer

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 464 KB
• Volume: 36
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490360410

✦ Synopsis

0-2A progenitor cells were grown in medium containing either 1% or 10% fetal calf serum (FCS) for 4 weeks. The cells in 1% FCS were 75% oligodendrocytes by 3 weeks in culture. The cell population was so overgrown with astrocytes in the 10% medium that an accurate estimate of cell number could not be made. The activities of glycerophosphorylcholine phosphocholine phosphodiesterase (GPC-PC-PdE j, p-nitrophenylphosphorylcholine phosphodiesterase (pNPPC-PC-PdE), and ceramide UDP galactose galactosyl transferase (CGalT) were barely detectable in the cells grown in 10% FCS. The activities of these 3 enzymes were low in the cells grown in 1 % FCS for the first 2 weeks and then all 3 increased manyfold. These observations reinforce the evidence previously accrued showing that these two phosphodiesterase activities (GPC-PC-PdE and pNPPC-PC-PdE) are markers of oligodendroglial cells as well as myelin. In contrast, glycerophosphorylcholine choline phosphodiesterase (GPC-C-PdE) activities were present in cells grown in both 1% and 10% FCS.