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Glutamate receptor-linked changes in membrane potential and intracellular Ca2+ in primary rat astrocytes

✍ Scribed by M.O. Kristian Enkvist; Irma Holopainen; Prof. Karl E.O. Åkerman

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
609 KB
Volume
2
Category
Article
ISSN
0894-1491

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✦ Synopsis

Kainate-, quisqualate-and glutamate-induced depolarization and mobilization of intracellular Ca2+ was determined in primary cultured astrocytes using the fluorescent probes DiBa-C,-(3) and fura-2, respectively. All three receptor agonists depolarized the cells in a Na+-dependent manner and increased the intracellular Ca2+ concentration. The glutamate-and quisqualate-induced increase in cytosolic Ca2+ was only partially inhibited by removal of extracellular Ca2+, whereas the response to kainate was totally dependent on extracellular Ca2+. The mechanisms for depolarization and increases in cytosolic Ca2+ appeared to be independent of each other, as extracellular Ca2+ removal or intracellular Ca2+ buffering with entrapped BAPTA did not affect the depolarization. Removal of extracellular Na+ did not affect the agonist-induced increase in Ca2+. If quisqualate was added after kainate, the cells were hyperpolarized in a Ca2'and K+-dependent manner. This could be due to effects on a Ca2+-dependent K+ channel, the effects of which are normally hidden by the greater effect on Na+ channels as a response to quisqualate.

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