Assay of Tubulin Acetyltransferase Activ
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R.A. Lloyd; S. Gentleman; G.J. Chader
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Article
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1994
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Elsevier Science
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English
โ 388 KB
An assay for alpha-tubulin acetyltransferase (TAT) activity based on affinity isolation of labeled acetylated alpha-tubulin was developed for use with crude subcellular fractions. Microtubules were polymerized and immobilized on an anti-alpha-tubulin-agarose and then incubated with the subcellular f