Glutamate dehydrogenase activity in normal and vitrified plants ofAgave tequilanaWeber propagated in vitro
✍ Scribed by Lizbeth Castro-Concha; Victor M. Loyola-Vargas; José L. Chan; Manuel L. Robert
- Book ID
- 104620737
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 366 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
Agave tequilana stem explants were used to produce adventitious shoots under a set of different water potentials induced by different concentrations of gelrite in the medium. At high water potentials all shoots were vitrified; as the medium water potential became more negative the degree of vitrification decreased but the number of shoots per explant also diminished. The enzymes NADH and NAD-GDH (EC. 1.4.1.2) were measured along the water potential gradient. GDH activity was high in the non-vitrified tissues and decreased significantly in the vitrified ones.