Glutamate-activated ionic currents in cultured astrocytes from trout: Evidence for the occurrence of non-N-methyl-D-aspartate receptors
✍ Scribed by T. Clasen; G. Jeserich; T. Krüppel
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 782 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0360-4012
No coin nor oath required. For personal study only.
✦ Synopsis
Glutamate-induced currents were recorded from cultured trout astrocytes with the whole-cell variation of the patch-clamp technique. Ninety percent of the tested cells were directly depolarized by the amino acid neurotransmitter in a concentration-dependent manner. The depolarizing effect was due to an inward current that reversed near 0 mV and was accompanied by a noise increase, indicating the opening of an ion channel. Ion substitution experiments revealed that the glutamate-induced current was mainly carried by sodium ions but not chloride or calcium ions. The glutamate-induced response could be mimicked by the neuronal glutamate receptor subtype agonists kainate and quisqualate, while N- methyl-D-aspartate was without detectable effect.