Glucosyltransferase activity in a water extract of maize pollen

✍ Scribed by R. L. Larson; Cynthia M. Lonergan

• Book ID: 104750255
• Publisher: Springer-Verlag
• Year: 1972
• Tongue: English
• Weight: 193 KB
• Volume: 103
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00386709

✦ Synopsis

Glucosyltransferase activity was extracted from maize pollen in distilled water. The enzymatic reaction required UDP-glucose, mercaptoethanol and Ca(++), and had a pH optimum at 8.2. Either kampferol or quercetin served as a substrate. Michaelis-Menten constants obtained were 0.6×10(-4) M for quercetin and 0.74×10(-3)M for UDP-glucose. Ammonium-sulfate precipitation of the enzyme gave a 4fold purification.