Glucose tolerant and thermophilic β-glucosidases from yeasts

✍ Scribed by Badal C. Saha; Rodney J. Bothast

• Book ID: 104633217
• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 237 KB
• Volume: 18
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00128671

✦ Synopsis

Forty-eight yeast strains belonging to the genera Candida, Debaryomyces, Kluyveromyces and Pichia (obtained from the ARS Culture Collection, Peoria, IL) were screened for production of extracellular glucose tolerant and thermophilic B-glucosidase activity using p-nitrophenyl-B-D-glucoside as substrate. Enzymes from 15 yeast strains showed very high glucose tolerance (< 50% inhibition at 30%, w/v glucose). The optimal temperatures and pH for these f~-glucosidase activities varied from 30 to 65°C and pH 4.5 to 6.5. The D-glucosidases from all these yeast strains hydrolyzed cellobiose.