Galactose metabolism in the red cells shows marked interspecies and even intraspecies variations. Red cells of guinea pig, dog and a group of rabbits metabolize galactose to a higher extent than those of other species, including human. In the rabbit, the difference in red cell galactokinase activity
Glucose metabolism of mammalian erythrocytes
β Scribed by John W. Harvey; J. J. Kaneko
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 444 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0021-9541
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β¦ Synopsis
Abstract
Under physiological conditions, erythrocytes of the horse metabolized 638 Β± 37 (Β± SE) nmoles glucose/ml cells/hr at 37Β°C compared to 942 Β± 31 for the cat, 1,329 Β± 44 for the dog, and 1,485 Β± 43 for man. On an absolute basis, pentose phosphate metabolism was similar between species, with species differences in erythrocyte glucose utilization attributable to differences in EmbdenβMeyerhof pathway metabolism. By examining pentose phosphate pathway recycling, it appears that some functional compartmentation exists within erythrocytes.
π SIMILAR VOLUMES
A method using 13C isotopes and GCMS/SIM was developed and was used to study glucose metabolism in several mammalian cell lines. Using [U-13C6]glucose, the 13C isotopomer distributions in glucose, lactic acid, glutamic acid, aspartic acid, and gamma-aminobutyric acid produced by the cells were deter