Glucose in “chemically pure” (C. P.) galactose: Its determination and elimination; Action of glucose oxidase on galactose
✍ Scribed by Don Scott; Frank E. Hammer
- Publisher
- Elsevier Science
- Year
- 1962
- Tongue
- English
- Weight
- 223 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Glucose aerodehydrogenase, commonly referred to as fungal glucose oxidase, exhibits a remarkable specificity for P-n-glucopyranose, oxidizing it to gluconic acid. This property has long been used for the selective "removal" of glucose from many foods (1, 2) and for the determination of glucose (3-9). Of approximately 50 carbohydrates tested (10, II), only 6 were reported to have a measurable action as substrate. 2-Deoxy-n-glucose was oxidized at 10% the rate of glucose, while the balance ranged from 1.9% for 4,6-dibenzylglucose to 0.14% for galactose. Considerable discussion has taken place in the literature as to whether these actions have been due to glucose impurities, contaminating enzymes, or an actual effect of the glucose oxidase (10, 12). The presence of a small glucose contaminant in galactose prepared from lactose might be anticipated. This work was undertaken to determine whether the action on galactose was real or an artifact, and, if an artifact, to free galactose of this glucose impurity.