Glucose excretion by the symbioticChlorellaofSpongilla fluviatilis

✍ Scribed by Andrea Fischer; Doris Meindl; Eckhard Loos

• Book ID: 104753794
• Publisher: Springer-Verlag
• Year: 1989
• Tongue: English
• Weight: 512 KB
• Volume: 179
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00393696

✦ Synopsis

Chlorella sorokiniana strain 211-40c, a symbiotic Chlorella isolated from a freshwater sponge, excreted between 3% and 5% of assimilated 14C02 as glucose in the light, with a pH optimum around 5. This percentage increased when the illuminance was lowered (to 15% at 200 lx). Release of [14C]glucose continued in the dark and could be inhibited by the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Net efflux of glucose occurred even at a concentration ratio of extracellular/intracellular glucose of 4. This, together with the sensitivity to FCCP, is taken as evidence for active transport. Exogenous [14C]glucose was taken up by the cells under conditions of net glucose efflux, showing uptake and excretion to take place simultaneously.