Glucose Dehydrogenase Based Bioelectrode Utilizing a Synergistic Scheme of Substrate Conversion

✍ Scribed by Juozas Kulys; Irina Bratkovskaja

• Publisher: John Wiley and Sons
• Year: 2012
• Tongue: English
• Weight: 229 KB
• Volume: 24
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.201100474

✦ Synopsis

Abstract

A Bioelectrode utilizing a synergistic scheme of substrate conversion was built using glucose dehydrogenase from Acinetobacter calcoaceticus immobilized on the surface of a graphite electrode. At saturated glucose concentration the bioelectrode responded to the low reactive substrate hexacyanoferrate(III) with a sensitivity of 0.0035 µA/µM cm^2^. The response of the bioelectrode increased up to the 3.4×10^4^ fold in the presence of high reactive organic electron acceptors (mediators). The increase of the response depended on the concentration of the mediators and their chemical nature. The sensitivity of the bioelectrode to mediators reached 7.3–77 µA/µM cm^2^. The comparison of the bioelectrode sensitivity with kinetic parameters of enzyme action in homogeneous solution revealed good correlation between the sensitivity of the bioelectrode and the predicted value from the kinetic scheme of the reactivity of mediators. This confirms a synergistic scheme of bioelectrode action.