Glucose 6-phosphate dehydrogenase in rainbow trout

✍ Scribed by Stephen D. Cederbaum; Akira Yoshida

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 826 KB
• Volume: 14
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00484764

✦ Synopsis

Electrophoretic analysis of glucose 6-phosphate dehydrogenase from liver and blood of rainbow trout revealed a complex series of bands, which could differ between fish. The partial interconvertible nature of these bands was demonstrated with enzyme that had been incompletely inactivated at pH S.4. In a single population of 40 fish, a homozygote and a heterozygote.for an eleetrophoretie variant allele were found. We suggest that G6PD in rainbow trout liver and blood is determined by two alleles at a single locus, with posttranslational modification responsible for the complex electrophoretie patterns seen. The basis for this variation appears to be N A D H binding to the protein molecule. Another variant and other properties of the enzyme are described.