Glucose-6-phosphate dehydrogenase was purified to homogeneity from testes and kidneys of the inbred strain of mice (DBA/2J) by a simple two-step affinity column procedure. This involved the sequential application of 8-(6-aminohexyl)-amino-AMP- and -2', 5'-ADP-Sepharose columns and biospecific elutio
Glucose-6-Phosphate and UDP-D-Glucose Dehydrogenases: Possible Markers of Vascular Differentiation
✍ Scribed by P. B. Gahan; A. McGarry; L. Wang; T. Doré; D. F. Carmignac
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 208 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
A quantitative cytochemical study of intact root apices and wounded roots of Pisum sativum, and of cotyledons from Solanum aviculare induced to form vessels from mesophyll cells, has demonstrated a sharp increase in glucose-6-phosphate dehydrogenase activity at a very early stage in the differentiation of the vascular tissues. However, UDP-D-glucose dehydrogenase activity appears to increase only at a time corresponding to the initiation of secondary cell wall events. Data from the callus cultures from Malus M27 and Cox endosperm indicated that glucose-6-phosphate dehydrogenase activity changes may be a possible marker for determining between the regenerability and recalcitrance of calluses.
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A total of 3000 men living in Yamaguchi were screened for glucose-6-phosphate dehydrogenase (G6PD) deficiency using Beutler's spot test and three types of starch gel electrophoresis. These electrophoresis used a phosphate buffer system at pH 7.0, a TRIS-EDTA-borate buffer system at pH 8.6, and a TRI