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Glucose-6-Phosphatase in Tubular Endoplasmic Reticulum of Hepatocytes

โœ Scribed by Rosen, S. I.; Kelly, G. W.; Peters, V. B.

Book ID
121176176
Publisher
American Association for the Advancement of Science
Year
1966
Tongue
English
Weight
701 KB
Volume
152
Category
Article
ISSN
0036-8075

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A simple procedure is presented for the enzymatic preparation of [2-3H]mannose 6-phosphate (Man 6-P) with purified yeast hexokinase and unlabeled ATP. The enzymatically synthesized [2-3H]Man 6-P is utilized as the radiolabeled substrate in a new rapid assay for glucose 6-phosphate (Glc 6-P) phosphat