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Gluconate catabolism in cowpea rhizobia: evidence for a ketogluconate pathway

✍ Scribed by Mark D. Stowers; Gerald H. Elkan

Book ID: 104770510
Publisher: Springer
Year: 1984
Tongue: English
Weight: 662 KB
Volume: 137
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00425799

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✦ Synopsis

Enzymatic and radiorespirometric analysis of several strains of cowpea rhizobia revealed the presence of key enzymes of the Entner-Doudoroff (ED) pathway with the operation of the hexose cycle for the dissimilation of gluconate. These bacteria lack the oxidative pentose phosphate (PP) pathway when grown on gluconate. Gluconate-grown cells possessed an operational tricarboxylic acid (TAC) cycle. Enzymes of an ancillary pathway, the ketogluconate (KG) pathway for gluconate catabolism were detected. The presence of this pathway was confirmed by techniques of thinlayer chromatography and radiorespirometry.

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