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Global Kinase Screening. Applications of Frontal Affinity Chromatography Coupled to Mass Spectrometry in Drug Discovery

✍ Scribed by Slon-Usakiewicz, Jacek J.; Dai, Jin-Rui; Ng, William; Foster, J. Estelle; Deretey, Eugen; Toledo-Sherman, Leticia; Redden, Peter R.; Pasternak, Andrew; Reid, Neil


Book ID
125941100
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
156 KB
Volume
77
Category
Article
ISSN
0003-2700

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Frontal Affinity Chromatography Coupled
✍ Boyan Zhang; Monica M. Palcic; David C. Schriemer; Gerardo Alvarez-Manilla; Mich 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 136 KB

Frontal affinity chromatography coupled online to mass spectrometry (FAC/MS) has previously been used to estimate binding constants for individual protein ligands present in mixtures of compounds. In this study FAC/MS is used to determine enzyme substrate kinetic parameters and binding constants for