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glnA mutations define the structural gene for glutamine synthetase inAspergillus

✍ Scribed by E. V. Cornwell; D. W. MacDonald

Publisher: Springer-Verlag
Year: 1984
Tongue: English
Weight: 323 KB
Volume: 8
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00405429

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✦ Synopsis

Five allelic glutamine auxotrophs of Aspergillus have been assayed for glutamine synthetase activi- ty. The amounts of glutamine synthetase activity present in these strains were proportional to their ability to grow in the absence of L-glutamine, whereas the amount of 3,-glutamyl transferase did not correlate with the amount of synthetase or with the growth properties. Two out of this sample of mutants have been shown to have a glutamine synthetase with a K m for L-glutamate which differs from the wild type.

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