GLC of trimethylsilyl derivatives of pantothenyl alcohol and pantothenates

✍ Scribed by A. R. Prosser; A. J. Sheppard

• Book ID: 102404048
• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 385 KB
• Volume: 60
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600600622

✦ Synopsis

The trimethylsilyl ester-ethers of pantothenic acid, calcium pantothenate, and sodium pantothenateand the trirnethylsilyl ethers of pantothenyl alcohol were prepared readily and quantitatively by using a mixture of bis(trimethylsilyl)acetamide, trimethylchlorosilane, trimethylsilylimidazole, and dimethyl sulfoxide.

GLC column packings of 5 % SE-30 and 3 OV-1 were demonstrated to yield symmetrical, resolved peaks for the trimethylsilyl derivatives. Quantitatively, 25 ng. of the trimethylsilyl derivatives, based on the starting materials, was effectively measured with the flame-ionization detector; GLC responses were linear as the quantity of the compound increased. The structures and molecular weights of the derivatives were confirmed by NMR and mass spectrometry.