GLC determination of acetohexamide and hydroxyhexamide in biological fluids

✍ Scribed by J. W. Kleber; J. A. Galloway; B. E. Rodda

• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 404 KB
• Volume: 66
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600660507

✦ Synopsis

A sensitive and specific GLC assay was developed for acetohexamide and hydroxyhexamide, its major metabolite, in plasma and urine. The assay uses tolbutamide as a mass internal standard. Compounds are extracted from acidified plasma or urine with toluene, converted to methylated derivatives with dimethyl sulfate, and measured by GLC using a flame-ionization detector. With GLC-mass spectrometry, the compounds measured are the N-methylsulfonamides resulting from GLC pyrolysis. Plasma and urine data are presented from a bioavailability study to demonstrate the utility of this method.