GLC analysis of ergonovine maleate

✍ Scribed by David L. Sondack

• Book ID: 102408866
• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 410 KB
• Volume: 63
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600630420

✦ Synopsis

A method is described for the quantitative determination of ergonovine maleate by GLC. Data are presented to show that this method provides the quantitative and semiquantitative results of the USP colorimetric assay and TLC in a single determination. Favorable quantitation is achieved by using brucine as internal standard. The sample is dried in the dark, derivatized with N-trimethylsilyldiethylamine qnd N-trimethylsilylimidazole in pyridine, and chromatographed on a column containing a nonpolar methyl silicone liquid phase. Degradation of the sample by preanalytical preparation (pH changes, air, heat, and light) are thereby minimized.

Keyphrases Ergonovine maleate-GLC analysis GLC-analysis, ergonovine maleate

It is generally recognized that the chemical purity of ergonovine (I) maleate is affected by oxygen, light, temperature, and extremes in pH (1-7). The chemi-cal nature of the degradation products (6, 7) may cause falsely high values when assays are performed by the colorimetric method described in USP XVIII