Glass capillary gas-chromatographic analysis of free amino acids in biological microenvironments using electron capture or selected ion-monitoring detection
✍ Scribed by Göran Bengtsson; Göran Odham; Gunilla Westerdahl
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 916 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Studies on analysis of free animo acids using a support-coated, open-tube capillary column, and electron-capture detection or selective ion monitoring have been performed on samples from biological microenvironments. For most amino acids the detection limit was found to be less than 1 pg. The preparation of the support-coated open-tube capillary column is described as well as the gas-chromatographic conditions for direct injection and temperature-programmed separation of the N-heptafluorobutyryl isobutyl ester derivatives. Electron-capture detection and selected ion monitoring are compared with respect to linearity and sensitivity and the bases for the greater sensitivity of electron-capture detection compared with flame-ionization detection using halogenated derivatives is discussed. Applications of the gas-chromatographic method for analysis of free amino acids in environments deliberately chosen very small are demonstrated.