✦ LIBER ✦

Glass beads as a solid matrix in in vitro study of the role of polyamines in cold hardiness of white clover

✍ Scribed by Kenneth MacLeod; Jerzy Nowak

Book ID: 104620734
Publisher: Springer
Year: 1990
Tongue: English
Weight: 691 KB
Volume: 22
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00043686

No coin nor oath required. For personal study only.

✦ Synopsis

Glass beads were used as an alternative to agar in the study of the role of polyamines in cold hardiness of white clover. Plantlet growth performance, in vitro hardening and cold stress tolerance were similar on both the agar solidified medium and the liquid medium in glass beads matrix. Glass beads allowed media exchange without plant transfer and an easy monitoring of the uptake of putrescine synthesis inhibitor, ~4C difluoromethylornithine from the medium. The matrix is recommended in in vitro studies of whole plant physiology, screening procedures and bioassays where media exchange and/or uniform application of a selection pressure is required. There is also 60% saving on media components and the beads can be re-used after acid wash.

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