✦ LIBER ✦
Geschlechtsbestimmung an Leichenmaterial durch Nachweis spezifischer Nukleotid-Sequenzen des Y-Chromosoms nach DNA-Spaltung
✍ Scribed by Nowak, R. ;Fink, T.
- Publisher
- Springer-Verlag
- Year
- 1986
- Tongue
- English
- Weight
- 926 KB
- Volume
- 97
- Category
- Article
- ISSN
- 1437-1596
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✦ Synopsis
A gene technological method of sex determination in cadaverous material is reported. The samples were taken from a child's corpse, which was nearly completely skeletonized after 1 year in water. From cells of the bone marrow the DNA was isolated and digested by restriction enzymes. A defined fragment of 2.12kb length was cleaved off by the endonuclease HaeIII in the presence of Y chromosomes. After agarose-gel electrophoresis of the DNA fragments, the specific sequence was detected by hybridization with the cloned, radioactively labelled complementary plasmide pHY2.1.