Microinjection of the Minos transposon is the only reported technique for generating stable transgenic lines in the cosmopolitan ascidian, Ciona intestinalis. To establish a more amenable method for generating stable transgenic Ciona, we examined the possibility of using electroporation of DNA into
Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis
β Scribed by Yasunori Sasakura
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 206 KB
- Volume
- 236
- Category
- Article
- ISSN
- 1058-8388
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The ascidian Ciona intestinalis is one of the model organisms of choice for comparative investigations of chordate development and for unraveling the molecular mechanisms underlying morphogenesis and cell fate specification. Taking advantage of the availability of various genetically encoded fluores
## Abstract Germline transgenesis with a Tc1/__mariner__ superfamily __Minos__ transposon was achieved in the ascidian __Ciona intestinalis__. Transgenic lines that express transposases in germ cells are very useful for remobilizing transposon copies. In the present study, we created transposaseβex
## Abstract Summary: The central and peripheral nervous systems (CNS and PNS) of the ascidian tadpole larva are comparatively simple, consisting of only about 350 cells. However, studies of the expression of neural patterning genes have demonstrated overall similarity between the ascidian CNS and t