Germination of pollen grains and growth of pollen tubes were studied to determine the cause of barreness in crosses among annual Cicer species. In vivo and in vitro time-course studies and fluorescent microscopy revealed no pollination incompatibility among the selfs, crosses and reciprocals of C. a
โฆ LIBER โฆ
Germination in vitro of ryegrass pollen grains
โ Scribed by B. S. Ahloowalia
- Publisher
- Springer
- Year
- 1973
- Tongue
- English
- Weight
- 344 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0014-2336
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โฆ Synopsis
Pollen grains of diploid and tetraploid Italian, perennial and hybrid ryegrass (Lolium spp.) germinated on 20 % sucrose solution with 10 ppm each of CaCI 2 , 1131303 and EDTA . Pollen tubes emerged within 2-3 minutes after sowing and completed full growth within 1 2' hours. The tube elongation showed the expected linear growth rate . The tubes of the haploid pollen grains grew faster (3 .9 ยตm/minute) than that of the diploid (3 .3 ยตm/minute) . Diploid pollen grains germinated better on 25 % than on 20% sucrose solution. Up to 25% of the diploid pollen produced two tubes .
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