Geographie variation of α-amylase, β-amylase, β-glucanase, pullulanase and chitinase activity in germinatingHordeum spontaneumbarley from Israel and Jordan
✍ Scribed by H. Ahokas; L. Naskali
- Book ID
- 104631429
- Publisher
- Springer Netherlands
- Year
- 1990
- Tongue
- English
- Weight
- 440 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0016-6707
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✦ Synopsis
The enzyme activities of c~-amylase,/3-amylase,/3-glucanase, pullulanase and chitinase were determined in extracts of wild barley (Hordeum vulgare ssp. spontaneum) germinated for five days under axenic standard conditions. The material comprises 257 accessions, for 242 of which the botanical territory of origin in Israel or Jordan is known. The enzyme activities based on soluble protein in the extracts showed significant differences (P < 0.001) among the eleven territories. The territorial moisture parameters mostly correlate with the enzyme activities. As determined by one gene or oligogenes, the significant territorial differences and the correlation with moisture are thought to reflect natural selection of genes responsible for favourable activity, or of genes linked to the enzyme coding loci, or in a coadaptive manner, of physiologically allied genes. Genes for high/3-glucanase activity at germination are probably coadaptive with genes for high/3-glucan content of the grain. The generally low starch content of wild barley grains probably makes any high c~-amylase activity unnecessary at the germination stage. An inverse relationship appears between/3-glucanase and chitinase activity; these two enzymes are also pathogenesis related proteins.