Geographic origin of the Y Chromosomes in “old” inbred strains of mice

✍ Scribed by Priscilla K. Tucker; Barbara K. Lee; Barbara L. Lundrigan; Eva M. Eicher

• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 785 KB
• Volume: 3
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00292153

✦ Synopsis

Six distinct Y Chromosomes (Chr) were identified among 39 standard inbred strains of mice with five probes that identified Y Chr-specific restriction fragments on Southern blots. Three Y Chr types, distributed among 31 strains, were of Asian Mus musculus origin. The remaining three Y Chr types, distributed among eight strains, were of M. domesticus origin. The Asian source of the M. musculus Y Chr was confirmed by determining the DNA sequence of 221 bp from an open reading frame within the Sry (sex determining region Y) gene (Gubbay et al., Nature 346: 245-250, 1990) in three inbred strains (C57BL/6J, AKR/J, and SWR/J) and comparing the sequence to the homologous sequences derived from wild caught European and Asian M. musculus males. These data indicate that a minimum of six male mice contributed to the formation of the old inbred strains.