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Genotypic and phenotypic analysis of the polymorphic thiopurine S-methyltransferase gene (TPMT) in a European population

✍ Scribed by Catherine Spire-Vayron de la Moureyre; Hervé Debuysere; Bruno Mastain; Elizabeth Vinner; Delphine Marez; Jean-Marc Lo Guidice; Dany Chevalier; Serge Brique; Kokou Motte; Jean-Frédéric Colombel; Dominique Turck; Christian Noel; René-Marc Flipo; Annie Pol; Michel Lhermitte; Jean-Jacques Lafitte; Christian Libersa; Franck Broly


Book ID
110003916
Publisher
Nature Publishing Group
Year
1998
Tongue
English
Weight
331 KB
Volume
125
Category
Article
ISSN
0007-1188

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To detect mutations in the thiopurine S-methyltransferase gene (TPMT), we have developed a strategy based on single-strand conformation polymorphism (SSCP) analysis of the gene amplified by polymerase chain reaction (PCR). The sensitivity of the method was first evaluated by analyzing DNA samples fr