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Genotypic and phenotypic analysis of the env gene from South African HIV-1 subtype B and C isolates

✍ Scribed by Florette K. Treurnicht; Tracey-Lee Smith; Susan Engelbrecht; Matilda Claassen; Brenda A. Robson; Michelle Zeier; Estrelita Janse van Rensburg

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
88 KB
Volume
68
Category
Article
ISSN
0146-6615

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✦ Synopsis

The objective of the study was to assess the genotypic and phenotypic properties of 18 viral strains from human immunodeficiency virus-1 (HIV-1) positive patients and to identify subtype C isolates for vaccine design strategies. All the isolates were non-syncytium-inducing (NSI) in both the primary and MT-2 cell cultures. The amino acid charge of the V3 loop correlated with the NSI phenotype of the strains. The V3 competitive peptide enzyme immunoassay and DNA sequencing of the partial gp120 region gave concordant results on the 15 subtype C strains, whereas the three B genotypes gave a positive to B, a nonreactive to B, and a dual reaction to the B-D peptides, respectively. Sixteen of the isolates used only CCR5 as coreceptor whereas two isolates made use of additional coreceptors including CXCR4. In summary, all our subtype C isolates are NSI phenotypically and almost all of them use CCR5 exclusively as their coreceptor.

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