Genotype diversity of group A rotavirus strains in children with acute diarrhea in urban Burkina Faso, 2008–2010

✍ Scribed by Isidore J.O. Bonkoungou; Susan Damanka; Idrissa Sanou; Fidèle Tiendrébéogo; Sheick O. Coulibaly; Fabienne Bon; Kaisa Haukka; Alfred S. Traoré; Nicolas Barro; George E. Armah

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 173 KB
• Volume: 83
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.22137

✦ Synopsis

Abstract

In this study, the diversity of G and P genotypes of rotavirus strains in Burkinabe children were examined. Between November 2008 and February 2010, 447 stool samples were collected from children <5 years of age with acute diarrhea visiting hospital in Ouagadougou. Group A rotavirus was previously detected in 151/447 (33.8%) of the samples tested by an immunochromatographic test and these samples were now tested further for rotavirus G and P genotypes by RT‐PCR. Of these, the rotavirus type genes were amplified by RT‐PCR for 140/151 (92.7%) samples and G and P genotypes were successfully determined for 81 (57.9%) and 130 (92.9%) samples, respectively. The most prevalent G genotypes were G1, 34/140 (24.3%), and G9, 21/140 (15%), while the predominant P genotypes were P[6], 56/140 (40%), and P[8], 54/140 (38.6%). Among the single infections, 63/140 (45%), the predominant G/P combinations were: G1P[8] (33%), G9P[8] (29%), and G2P[6] (14%). The unusual strains G1P[9] (3%), G12P[6] (3%), G10P[6] (2%), and G2P[8] (2%) were also detected. In a high number of strains 61/140 (43.6%), the G genotype could not be determined and mixed infections were determined in 17/140 (12.1%) of strains identified. This study highlights the high diversity and presence of unusual rotavirus strains in children in Burkina Faso. J. Med. Virol. 83:1485–1490, 2011. © 2011 Wiley‐Liss, Inc.