𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Genomic variation among herpes simplex virus type 1 strains: Virus DNA analysis of isolates from saudi patients

✍ Scribed by Dr. Mohammed N. Al-Ahdal; George Kessie; Mohamed A. Taha; Fahad J. Al-Shammary; Mohamed Ettayebi

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
597 KB
Volume
38
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Fifty‐two clinical isolates of herpes simplex virus type 1 (HSV‐1) from Saudi Arabian patients were analysed by restriction endonuclease digestion of the virus DNA using the enzymes Hindlll and BarnHI, followed by hybridization with 32P labelled DNA of laboratory strain F. Of the isolates, 17 were resolved into four distinct cleavage patterns with __Hind__III restriction enzyme. The remaining 35 strains had the same cleavage pattern as the standard HSV‐1‐F. Further investigation of the 52 isolates with BarnHI, which is a multicut enzyme and therefore capable of higher resolution, differentiated 47 of the 52 isolates and were assigned into nine cleavage groups. Comparing our findings with similar studies reported elsewhere suggest geographic clustering of HSV‐1 strains. Fragments giving rise to the observed DNA polymorphism were mapped to the unique region of the long and short segments of the genome. © 1992 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Genotyping of herpes simplex virus type
Genotyping of herpes simplex virus type 1 strains isolated from ocular materials of patients with herpetic keratitis
✍ Kenichi Umene; Tomoyuki Inoue; Yoshitsugu Inoue; Yoshikazu Shimomura 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 86 KB

## Abstract Herpes simplex virus type 1 (HSV‐1) is the etiological agent of herpetic keratitis. The epithelial form (“epithelial keratitis”) is attributed mainly to destruction of the epithelium through active viral replication within the epithelium. The stromal form (“stromal keratitis”) is associ

Serologic and genotypic analysis of a se
Serologic and genotypic analysis of a series of herpes simplex virus type 1 isolates from two patients with genital herpes
✍ Kenichi Umene; Takashi Kawana; Yasuyuki Fukumaki 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 115 KB 👁 1 views

## Abstract Herpes simplex virus type 1 (HSV‐1) has been reported increasingly as a cause of genital herpes, although HSV‐1 is usually associated with oro‐labial herpes. In the present study, serum specimens and materials for viral isolation were obtained serially from two patients with recrudescen

Polymorphisms of thymidine kinase gene i
Polymorphisms of thymidine kinase gene in herpes simplex virus type 1: Analysis of clinical isolates from herpetic keratitis patients and laboratory strains
✍ Kudo, E.; Shiota, H.; Naito, T.; Satake, K.; Itakura, M. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 416 KB 👁 2 views

Drug-resistance of herpes simplex virus (HSV) is caused most frequently by mutation of the viral thymidine kinase (TK) gene. To elucidate the significance of detecting nucleotide changes of the TK gene for screening drug-resistant viruses, the frequency and variation of the genetic polymorphisms in

Genotypic analysis of sequential genital
Genotypic analysis of sequential genital herpes simplex virus type 1 (HSV-1) isolates of patients with recurrent HSV-1 associated genital herpes
✍ R.W. Roest; W.F. Carman; J. Maertzdorf; A. Scoular; J. Harvey; M. Kant; W.I. van 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 86 KB

## Abstract Clinical recurrences of Herpes simplex virus type 1 (HSV‐1)‐associated genital herpes are thought to be caused by reactivation of latent endogenous HSV‐1. However, the possibility of reinfection with exogenous HSV‐1 cannot be excluded. This study aimed to determine the incidence of geni