✦ LIBER ✦

Genomic instability occurs in colorectal carcinomas but not in adenomas

✍ Scribed by Joanne Young; Barbara Leggett; Corinne Gustafson; Michael Ward; Jeffrey Searle; Lesley Thomas; Ron Buttenshaw; Georgia Chenevix-Trench

Book ID: 102260075
Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 355 KB
Volume: 2
Category: Article
ISSN: 1059-7794
DOI: 10.1002/humu.1380020505

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✦ Synopsis

Genomic instability, as demonstrated by the presence of additional alleles at short tandemly repeated (STR) loci, has recently been observed in colorectal tumours from individuals with hereditary nonpolyposis colorectal cancer (HNPCC), and in some sporadic tumours. These neoplasms have been called replication error positive (RER+). In this study, we confirm the presence of genomic instability in a proportion of unselected colorectal carcinomas but find no evidence of instability in adenomas. We further report replication errors in a tetranucleotide sequence, and in STRs within two tumour suppressor genes. 108 colorectal adenocarcinomas and 46 adenomas were analysed for the presence of variant bands at 4-15 microsatellite markers. Seven (6.5%) of carcinomas were RER+, four of which originated from the proximal colon. Analysis of the adenomas and of matched adenoma-carcinoma and carcinoma-metastatic samples from four patients suggests that the replication errors may occur during the development of carcinomas but are rare in adenomas.

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