Genomic Imprinting: Methods and Protocols
β Scribed by Yong Cheng, Dasari Amarnath, Keith E. Latham (auth.), Nora Engel (eds.)
- Publisher
- Humana Press
- Year
- 2012
- Tongue
- English
- Leaves
- 295
- Series
- Methods in Molecular Biology 925
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting. This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers. Written in the highly successful Methods in Molecular Biologyβ’ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.β¦ Table of Contents
Front Matter....Pages i-xi
Front Matter....Pages 1-1
Front Matter....Pages 3-19
Front Matter....Pages 21-48
Front Matter....Pages 49-59
Front Matter....Pages 61-66
Front Matter....Pages 67-67
Front Matter....Pages 69-78
Back Matter....Pages 79-88
....Pages 89-134
β¦ Subjects
Human Genetics
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<p>Imprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning
Univ. of Bath, UK. Brings together the essential molecular, genetic, and embryological methods used in today's laboratories for the identification and analysis of imprinted genes. Each technique is described in detail. Outline format.
Univ. of Bath, UK. Brings together the essential molecular, genetic, and embryological methods used in today's laboratories for the identification and analysis of imprinted genes. Each technique is described in detail. Outline format.
<p>Imprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning