Genomic characterization of rotavirus strains obtained from hospitalized children with diarrhoea in Bangladesh

✍ Scribed by Shahina Tabassum; P. Shears; Prof. C. A. Hart

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 800 KB
• Volume: 43
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890430110

✦ Synopsis

Abstract

Faecal samples were obtained from 111 children hospitalized with acute watery gastroenteritis in a children's hospital in Bangladesh from January to December, 1989. Rotavirus was detected as the aetiologic agent in 35 (32%) of these patients. The electrophoretic pattern of ds‐RNA extracted from the rotaviruses showed 11 different migration patterns (six short and five long), as well as some short and long mixed profiles. All four major G serotypes were identified by amplification of a segment of the gene for VP7 using the polymerase chain reaction. A specific serotype could be assigned in 32 (91%) cases. Serotypes G1‐G4 accounted for 11%, 37%, 11%, and 23% of isolates respectively. Three (9%) mixed serotypes were identified by this method and were found to be mixtures of serotypes G1 and G4, G2 and G4, and G3 and G4. © 1994 Wiley‐Liss, Inc.