Understanding the actions of drugs and toxins in a cell is of critical importance to medicine, yet many of the molecular events involved in chemical resistance are relatively uncharacterized. In order to identify the cellular processes and pathways targeted by chemicals, we took advantage of the hap
Genome-wide generation of yeast gene deletion strains
β Scribed by Diane E. Kelly; David C. Lamb; Steven L. Kelly
- Publisher
- Hindawi Publishing Corporation
- Year
- 2001
- Tongue
- English
- Weight
- 114 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1531-6912
- DOI
- 10.1002/cfg.95
No coin nor oath required. For personal study only.
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Using PCR to construct disruption cassettes, null alleles of six genes have been created in Saccharomyces cerevisiae. In a FY1679 background, no defects were detected in any of the haploid deletion mutants with respect to growth, gross morphology, or mating. A diploid FY1679-derived delta ygl194c/de
With rapid advances in genotyping technologies in recent years and the growing number of available markers, genomewide association studies are emerging as promising approaches for the study of complex diseases and traits. However, there are several challenges with analysis and interpretation of such